Background: Accidental autoclaving of L-glutamine was found to facilitate the Agrobacterium infection of a non host plant like tea in an earlier study. In the present communication, we elucidate the structural changes in L-glutamine due to autoclaving and also confirm the role of heat transformed L-glutamine in Agrobacterium mediated genetic transformation of host/non host plants. Results: When autoclaved at 121°C and 15 psi for 20 or 40 min, L-glutamine was structurally modified into 5-oxo proline and 3-amino glutarimide (-amino glutarimide), respectively. Of the two autoclaved products, only -amino glutarimide facilitated Agrobacterium infection of a number of resistant to susceptible plants. However, the compound did not have any vir gene inducing property. Conclusions: We report a one pot autoclave process for the synthesis of 5-oxo proline and -amino glutarimide from L-glutamine. Xenobiotic detoxifying property of -amino glutarimide is also proposed. © 2011 Sandal et al; licensee BioMed Central Ltd.
CITATION STYLE
Sandal, I., Bhattacharya, A., Saini, U., Kaur, D., Sharma, S., Gulati, A., … Ahuja, P. S. (2011). Chemical modification of L-glutamine to alpha-amino glutarimide on autoclaving facilitates Agrobacterium infection of host and non-host plants: A new use of a known compound. BMC Chemical Biology, 11. https://doi.org/10.1186/1472-6769-11-1
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