Forced Degradation Studies on the Piperine-Based Benzene Sulfonylamidine: The Effect of Acid, Base, and Peroxide on Its Stability

Abstract

A research about the effect of acid, base, and peroxide on the stability of piperine-based benzene sulfonylamidine (PBSA) on the forced degradation studies utilizing High-Performance Liquid Chromatography (HPLC) as the detection method has been conducted. Having biological activity as anticancer, PBSA was derived from the modification of piperine by click-type reaction of thioamide and sulfinyl azide. It was treated in several conditions such as 0.1 N HCl, 0.1 NaOH, and 3% peroxide for 7 days with sampling on days 1 and 7 respectively. This method was evaluated on the SHIMADZU® HPLC system integrated with a PDA detector. The analysis was arranged in an isocratic separation mode in column C-18 (COSMOSIL®), 150 mm x 4.6 mm, column particle size: 5 μm. The mobile phase of methanol and water at a ratio result showed that PBSA was stable to acid, base and peroxide.