Simplifiedenzyme-linked immunosorbent assay for routine identification of Rhizobium japonicum antigens

Abstract

A simple, reliable, and flexible modification of the indirect enzyme-linked innumosorbent assay was developed for the identification of Rhizobium antigens from cultures and nodules. The procedure emphasizes efficient use of time and reagents, adaptability to variously equipped laboratories, and maintenance of sensitivity levels that are adequate for ecological studies.