Micropropagation of ophiorrhiza eriantha wight, through leaf explant cultures

Abstract

Leaf explants of Ophiorrhiza eriantha cultured in MS supplemented with the combination of NAA 4 mg/1 and BA 0.5 mg/1 induced higher callus growth. The maximum number of shoots were produced from callus on the MS supplemented with BA 5 mg/1. The regenerated shoots were transferred into the auxin containing medium for rooting and IBA 3 mg/1 supplemented medium produced maximum number of roots per shoot. Camptothecin (anticancer drug) was isolated from O. eriantha wild grown plant and in vitro regenerated plants, and was confirmed by LC-MS-MS. The camptothecin content in wild grown plant, callus and regenerated plants were quantified by HPLC system.