EKSTRAK ANTOSIANIN DARI UBI UNGU (Ipomoea batatas L.) SEBAGAI PEWARNA ALAMI PADA SEDIAAN APUSAN DARAH TEPI

Abstract

The peripheral blood smear (SADT) is a human blood cell that is made smear and then stained in a hematology examination to assess various elements of peripheral blood cells such as erythrocytes, leukocytes and platelets. One guarantee of the validity of the results of the SADT examination is the quality of the SADT coloring. This study aims to investigate blood morphology in thin blood smears using anthocyanin extract from purple sweet potato as a natural dye for Giemsa dye. This research is an experimental laboratory by treating and comparing the morphological features of blood (erythrocytes, platelets and leucocytes) in thin blood smears using giemsa staining and using alternative staining of anthocyanin extract from purple sweet potato. Samples in the study using venous blood collected in a vacuum tube using EDTA anticoagulant were then made as a thin blood smear preparation. The results of this study indicated that only erythrosies had a fairly good staining quality, while the quality of the platelets and leucocytes was not good. The morphology of erythrocyte cells that are round or oval and from the side is like a disc (bikonkaf) with a central acromia (pale color). Furthermore, several abnormalities of erythrocyte cell are obtained such as elliptocytes (ovalocytes), schistocytes (helmet cell) and burr cell so that the anthocyanin extract in purple sweet potato has the potential as an alternative stain for erythrocyte cells in peripheral blood smears. Keywords