Inflorescence and bunch architecture development in Vitis vinifera L.

Abstract

Inflorescence development from budburst to harvest was analysed in four cultivars of grapevine. Two cultivars with tight or compact bunches (Riesling, Chardonnay) and two with loose or open bunches (Exotic and Sultana) were selected to define differences in bunch development for future genetic analysis. A range of phenotypic characters for both inflorescence and shoot architecture were measured. Differences in the rate of rachis elongation rates were observed between tight and loose bunch cultivars commencing at the earliest stages of inflorescence development after budburst. At anthesis, five phenotypic characters showed significant differences between tight and loose cultivars: (1) total inflorescence length, (2) node number per inflorescence rachis, (3) combined length of two consecutive internodes of the rachis and (4) shoot node position at which the inflorescence was present and (5) mature tendril length. A quantitative estimate of bunch compactness was calculated at bunch maturity. Exotic and Sultana had significantly more open space than did compact bunch cultivars Riesling and Chardonnay. Comparison of flower number at anthesis and berry number at maturity indicated that the proportion of berries set was similar in all cultivars studied and, therefore, did not contribute to variability in bunch openness between cultivars. Internode length of the inflorescence rachis was the major trait responsible for inflorescence openness. Cellular studies using SEM, fluorescence microscopy and DNA content demonstrated that differences in rachis internode lengths were mostly associated with cell expansion.