Development and validation of RP-HPLC method for quantification of berberine in ethanol fraction of methanol extract and developed formulation of Tinospora cordifolia

Abstract

Simple and precise RP-HPLC method was developed for quantification of berberine in ethanol fraction of methanol extract of Tinospora cordifolia and its formulation. In this, separation was achieved on a HPLC System (Shimadzu) Luna, C18 column. As mobile Phase acetonitrile and water (40:60) were used with a flow rate of 1ml/min and maximum absorbance was found at 266nm. In this we obtained calibration curve in the range of 20 - 640 mg/mL. The slope was 68734, intercept was 20563 and correlation coefficient was found to be 0.999. The limit of detection was 0.8 and limit of quantification was 1.7. For berberine the method was validated according to ICH guidelines. Method was highly precise and accurate because it shows low relative standard deviation as well as good % recovery values. Quantity of berberine was found was found to be 282.3 ppm. In tablet of 325mg.