In vitro ultraviolet radiation effects on growth, chemical constituents and molecular aspects of Spathiphyllum plant

Abstract

Spathiphyllum is one of the best plants for indoor gardening as it is a rhizome and undemanding of light care. It is a member of lily family (Araceae). The plant itself should live for 10 years or more if not maltreated. Spthiphyllum cannifolium plants are used in clean air study for their ability to remove formaldehyde, benezyne, and CO2 from interior air. UV is divided into three wavelength ranges; UV radiation can have damaging effects on DNA and/or physiological processes and play central role in characterization of bioresponses. A large number of studies have been conducted to evaluate the potential consequences of an increase in UV-B radiation on many plants. In this investigation, plants treated with 45-min ultraviolet radiation (UV) recorded the highest values on growth parameter, anatomical structure, survival %, and proline %. Fifteen minutes of UV radiation exposure gave the second highest value of previous parameters. Conversely, exposure of plantlets to moderate dose (30 min of UV radiation exposure) recorded fewer values in previous mentioned parameters. Start codon targeted (SCoT) analyses exhibited 39 DNA bands which appeared by using seven primers. These bands were identified as 15 polymorphic and 24 monomorphic ones with 38.46% polymorphism. Twelve unique bands were identified in the resulted SCoT profile. The results were nearly in an ascending order with increasing of UV-B radiation exposure time. Increasing exposure time to 45 min increased significantly the values of vegetative growth, anatomical parameters, and proline percentage in most cases compared with control and other UV-B radiation treatments. In the contrary, 30 min recorded decreased in previous mentioned treatments. The SCoT (Start codon targeted) marker is helpful for possible distinguishing, identifying, and characterizing of UV treatments.