Two in vitro assays were performing in order to improve Aristotelia chilensis germination rate. Scarification was tested in the first assay and compare to a control. In the second assay, cold stratification was applied during 2, 4, 6 and 8 weeks at 4 °C in darkness, plus an unstratified control. Seeds were germinated on Petri dish containing water and agar (WA), and incubated for 60 days in a controlled atmosphere at 25 ± 2 °C using cold white fluorescent light tubes (50 μmol m-2 s-1 light intensity), under a photoperiod of 16 h light and 8 h of darkness. All treatments achieved higher average germination rates than the control. The highest germination rate was obtained with scarification (Gmáx, 92%) though the fastest growth was achieved with 8 weeks of stratification, reaching the lowest average germination time of 18 days. The results demonstrate that A. chilensis seeds present a moderate level of primary dormancy, both exogenous and endogenous.
CITATION STYLE
Rodríguez Beraud, M., Tampe Pérez, J., Hormazábal Vásquez, N., Araneda Durán, X., Tighe Neira, R., & Cárcamo-Fincheira, P. (2017). Efecto de la escarificación y estratificación sobre la germinación in vitro de Aristotelia chilensis (Molina) Stuntz. Gayana - Botanica, 74(2), 282–287. https://doi.org/10.4067/S0717-66432017000200282
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