Antibacterial, antivirulence and antifungal activity of silver nanoparticles synthesized using alkhal mother shae

Abstract

Silver nanoparticles were biosynthesized using alkhal mother shae. Nanoparticles were characterized using four methods. The results of the fourth methods proved that silver nanoparticles are spherical shape with a size ranging between 30-40 nm. AMS-AgNPs has antibacterial and antibiofilm activity against gram negative and gram positive tested bacteria, and it has antifungal and antivirulence activity against five types of fungi. Results declare the effect of AMS-AgNPs on the fungus, it can disrupt the integrity fungal cell wall, promoting the permeability and the leakage of the cell constituents, and eventually induce cell death, which is reached from the Congo red dye absorption test and the formation of swelling in the hyphae of the fungal. This study revealed that AMS-AgNPs can be used as an alternative medicin for pathogenic bacteria and fungi.