Salinity-imposed changes of some isozymes and total leaf protein expression in five mangroves from two different habitats

Abstract

A comparative account of the polymorphic expression of two antioxidative enzymes (Peroxidase and Superoxide dismutase), two hydrolyzing enzymes (Esterase and Acid phosphatase) and total proteins was estimated both qualitatively and quantitatively from the leaves of five mangroves (Bruguiera gymnorrhiza, Excoecaria agallocha, Heritiera fomes, Phoenix paludosa and Xylocarpus granatum) from two different habitats (in situ habitat of Sundarbans and their replicas from ex situ habitat in fresh water condition) and discussed. The higher amount of total protein occurred in fresh water plants compared to their Sundarbans counterparts. The increment of total protein ranged among 156% to 5.7%. Gel electrophoresis experiments revealed that in most of the cases there were extra numbers of protein bands expressed with relatively low molecular weight in saline habitat. In all salinity imposed plants, there were sharp increases in band intensity and the number of isoforms of investigated enzyme. Peroxidase increment in saline plants ranged from 257-139%. Similarly, SOD was estimated at 247-147% in saline habitats. Increments of Esterase and Acid phosphatase varied from 287-154% and 293-139%, respectively. © 2010 Taylor & Francis.