rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA

  • Won S
  • Cho S
  • Kim H
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Abstract

We quantitatively demonstrated that the rRNA operon outperformed the 16S rRNA and its V3-V4 regions in accuracy for both individual species identification and species-level microbial community analysis. Our findings can provide guidelines for selecting appropriate markers in the field of microbial research.

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Won, S., Cho, S., & Kim, H. (2024). rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA. Microbiology Spectrum, 12(11). https://doi.org/10.1128/spectrum.00931-24

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