Quantitative methods for genome-scale analysis of in situ hybridization and correlation with microarray data

Abstract

With the emergence of genome-wide colorimetric in situ hybridization (ISH) data sets such as the Allen Brain Atlas, it is important to understand the relationship between this gene expression modality and those derived from more quantitative based technologies. This study introduces a novel method for standardized relative quantification of colorimetric ISH signal that enables a large-scale cross-platform expression level comparison of ISH with two publicly available microarray brain data sources. © 2008 Lee et al.; licensee BioMed Central Ltd.