Successful tips of DNA extraction and PCR of plants for beginners

Abstract

DNA extraction and PCR are among the most fundamental, essential, and inevitable experiments in plant genetics and biotechnology. Despite their long history, these protocols are still improved by researchers to perform more efficient and more successful experiments. In this review, we will try to introduce the key to successful DNA extraction and PCR experiments, especially for beginners of genetic analyses, to avoid making easy mistakes which often result in great waste of time and reagent. These keys are related to how to correctly measure DNA amount/purity, how to extract DNA from 'difficult' plant species, and how to successfully amplify target DNA sequence from huge plant genomic DNA.