Enhanced transmembrane electron transfer in Shewanella oneidensis MR-1 using gold nanoparticles for high-performance microbial fuel cells

Abstract

Low efficiency of extracellular electron transfer (EET) is a major bottleneck in developing high-performance microbial fuel cells (MFCs). Herein, we construct Shewanella oneidensis MR-1@Au for the bioanode of MFCs. Through performance recovery experiments of mutants, we proved that abundant Au nanoparticles not only tightly covered the bacteria surface, but were also distributed in the periplasm and cytoplasm, and even embedded in the outer and inner membranes of the cell. These Au nanoparticles could act as electron conduits to enable highly efficient electron transfer between S. oneidensis MR-1 and electrodes. Strikingly, the maximum power density of the S. oneidensis MR-1@Au bioanode reached up to 3749 mW m−2, which was 17.4 times higher than that with the native bacteria, reaching the highest performance yet reported in MFCs using Au or Au-based nanocomposites as the anode. This work elucidates the role of Au nanoparticles in promoting transmembrane and extracellular electron transfer from the perspective of molecular biology and electrochemistry, while alleviating bottlenecks in MFC performances.