Effect of gamma irradiation on Jati Belanda (Guazuma ulmifolia L) against human cancer cell lines

Abstract

The herbal plants commonly used in Indonesia are Jati belanda (Guazuma ulmifolia L) which is commonly used as a medicine for high blood pressure and cancer. During the storage, to reduce contamination from bacteria, fungi, and other microorganisms can be used gamma irradiation. Gamma Irradiation Co-60 can be used as an alternative for preserving herbal plants. This study aimed to examine the effect of Co-60 (0 and 7.5 kGy) gamma-irradiation onG. ulmifolia plants on anti-cancer line properties (THP-1, K-562, and MCF-7). G.ulmifolia herb without irradiation (0 kGy) and gamma-irradiated 7.5 kGy using Co-60. The samples were macerated using ethanol as much as 2 replications. Cytotoxic tests were performed on ethanol extracts against line cancer cells (THP-1, K-562, and MCF-7) with varying concentrations of 5, 10, 20, 40, and 80 ¡ig / ml. The results showed that irradiation doses of 0 kGy and 7.5 kGy were very active in inhibiting K-562 cancer cells with IC5 0 respectively 10.94 |ig/ml and 16.74 |ig/ml. Gamma irradiation can be used as an alternative in preserving medicinal plants without reducing the anti-cancer properties from the G.ulmifolia.