Callus induction, indirect organogenesis and plantlet regeneration from different explants of vernonia anthelmintica (L.) willd

Abstract

Present study was conducted to standardize callus development and indirect organogenesis from different explants of Vernonia anthelmintica (L.) Willd. Among, the various hormonal combinations tested: Indole Acetic Acid (IAA) and BA and IAA and Kinetin combinations were found to be optimum for inducing callusing within a time span of 10 days. Best callus response was observed in 1.5 mg l−1 IAA and 1.5 mg l−1 BA, which produced white friable callus. Best indirect shoot organogenesis was observed in 4 mg l−1 BA and 6 mg l−1 kinetin. Elongated shoots when transferred on to half strength Murashige and Skoog (MS) Medium supplemented with Indole-3-butyric acid (IBA), rooting was observed. MS medium fortified with 2 mg l−1 IBA showed better rooting than all other concentrations tested. This concentration produced maximum number of roots and maximum percentage of rooting. Plantlets developed by indirect organogenesis of V. anthelmintica were successfully acclimatized to field conditions.