Evaluación de métodos para la separación eficiente de leucocitos

Abstract

In the use of diagnostic methods for malaria parasites, based on polymerase chain reaction (PCR) in whole blood, leucocyte presence frequently causes interference which can completely mask resuks.'ln order to avoid this problem, different methods were tested to eliminate leucocytes from whole blood, based on the density and size of blood components. This elimination procedure was controlled by DNA polymerase chain reaction using arbitraty primers (RAPD). This method's high sensitivity allowed us to accurately define that whole blood's filtration through a cellulose-sephadex G-25 column allgws the best elimination, compared to other matrices or separation methods such as ficoll, dextran or ficoll-dextran.