Diagnosis of human toxoplasmosis using toxoplasma gondii tachyzoites crude antigen of different origin

Abstract

In the present study, crude extract of Toxoplasma gondii tachyzoites were prepared from chicken local isolate and adopted for diagnosis of chicken toxoplasmosis by ELISA. The extract proved success in detection of Toxoplasma antibodies in 275 (78.12%) out of 352 examined free rang chicken serum samples. The important facet of the present study is adoption of crude chicken tachyzoites antigen in diagnosis of human toxoplasmosis, where it proved 100% sensitivity in the detection of Toxoplasma antibodies in all examined cases (61 samples) of human toxoplasmosis. Meanwhile, RH strain antigen, which used to be utilized in diagnosis of human toxoplasmosis, detected only 80% of chicken toxoplasmosis samples. By SDS-PAGE, chicken tachyzoites antigen resolved to 8 bands of molecular weights ranged from 30-116 kDa. Also, RH strain antigen revealed 10 bands with different molecular weights ranged from 23-166 kDa. The immunoreactive bands were detected by immunoblotting assay. One band of 116 kDa was detected in chicken antigen and 28 kDa in RH antigen by infected human sera. While, two bands with molecular weights 116 and 83 kDa and 116 and 30 kDa were detected in chicken tachyzoites and RH antigens, respectively using infected chicken sera. A band of 116 kDa was detected in chicken antigen using both sera, which is mainly responsible for diagnosis of chicken and human toxoplasmosis. In conclusion, chicken tachyzoites antigen based ELISA can be successfully utilized in diagnosis of chicken and human toxoplasmosis.