Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream Formulations

Abstract

An HPLC method for the simultaneous quantitative determination of betamethasone and clotrimazole in cream formulation has been developed. The method utilizes a reversed-phase C18 (250 X 4.0 mm) stationary phase, with a mixture of methanol-acetate buffer-acetonitrile (33:27:40, v/v) as a mobile phase, and spectrophotometric UV detection at 254 nm. The method has been validated for cream formulations containing betamethasone and clotrimazole with linear range of 0.025 to 0.075 mg/ml for betamethasone with a correlation coefficient of 0.9996, and linear range of 0.25 to 0.75 mg/ml for clotrimazole with a correlation coefficient of 1.000. The results demonstrated that this method is accurate, precise, specific, linear, reliable, sensitive, and fast.