Appearance of novel glucose-6-phosphate dehydrogenase isoforms in Chlamydomonas reinhardtii during growth on nitrate

Abstract

Extractable glucose-6-phosphate dehydrogenase activity is higher from N-limited Chlamydomonas reinhardtii cells than from N-sufficient cells. Native gels reveal that the isoform complexity varies depending on the form of N supplied. The isoforms associated with NO3- growth appear within 2 h of switching cells from NH4+ to NO3-.