Enhanced-latex-agglutination assay for C-reactive protein in serum, with use of a centrifugal analyzer

Abstract

This is an improved assay of C-reactive protein in serum, for use with the Baker 'Encore' centrifugal analyzer. Features of this assay include: 250-specimen throughout per hour, within-batch CV 2.2%, between-batch CV 2.7%, no antigen-excess problems up to 1000 mg/L, negligible interference from rheumatoid factor, and good correlation (r = 0.99) with radial immunodiffusion. The method is inexpensive and automated, involving no predilution steps. It can be adapted for use in a wide range of systems and can be used for single urgent estimations.