CRISPR/Cas Systems in Tissue Engineering: A Succinct Overview of Current Use and Future Opportunities

Abstract

Tissue engineering is a broad field that aims to restore or maintain the function of tissues and organs. Despite progress in the field, non-trivial obstacles have prevented the widespread adoption of tissue engineered products. Genome editing and synthetic biology approaches may be suitable strategies to tackle a number of these obstacles. Clustered regularly interspaced short palindromic repeats systems have revolutionized genome engineering and facilitated its adoption by many non-specialized laboratories, allowing tissue engineering laboratories to genetically modify cells for applications ranging from immune response circumvention to in vitro 3D micro tissue model development. This review presents a brief overview of the current use and future applications of clustered regularly interspaced short palindromic repeats systems in tissue engineering.