Establishment of cell lines from both myeloma bone marrow and plasmacytoma: SNU-MM1393-BM and SNU-MM1393-SC from a single patient

Abstract

Purpose. We tried to establish clinically relevant human myeloma cell lines that can contribute to the understanding of multiple myeloma (MM). Materials and Methods. Mononuclear cells obtained from MM patient's bone marrow were injected via tail vein in an NRG/SCID mouse. Fourteen weeks after the injection, tumor developed at subcutis of the mouse. The engraftment of MM cells into mouse bone marrow (BM) was also observed. We separated and cultured cells from subcutis and BM. Results. After the separation and culture of cells from subcutis and BM, we established two cell lines originating from a single patient (SNU-MM1393-BM and SNU-MM1393-SC). Karyotype of the two newly established MM cell lines showed tetraploidy which is different from the karyotype of the patient (diploidy) indicating clonal evolution. In contrast to SNU-MM1393-BM, cell proliferation of SNU-MM1393-SC was IL-6 independent. SNU-MM1393-BM and SNU-MM1393-SC showed high degree of resistance against bortezomib compared to U266 cell line. SNU-MM1393-BM had the greater lethality compared to SNU-MM1393-SC. Conclusion. Two cell lines harboring different site tropisms established from a single patient showed differences in cytokine response and lethality. Our newly established cell lines could be used as a tool to understand the biology of multiple myeloma.