Detection of Intracellular Adhesion (ica) and Biofilm Formation Genes in Staphylococcus aureus Isolates from Clinical Samples

Abstract

Background: The nosocomial infections that cause the establishment of biofilms on the embedded biomedical surfaces are the leading cause of sepsis and are often related to colonization of implants by Staphylococcus epidermidis. Materials and Methods: A total of 40 clinical S. aureus isolates were collected from Zabol, Iran. The ability of these strains to form biofilm was determined by microliter tissue culture plates. All clinical isolates were tested for the presence of icaA and icaD genes by Polymerase Chain Reaction (PCR). Results: The outcome of this study showed that from 40 isolates of Staphylococcus aureus, 12 (30%) of them were contained icaA gene and 8 (20%) isolates were positive for icaD gene and five (12.5%) isolates were contained both genes. Conclusion: The capability of S. aureus clinical isolates to form biofilm is different/diverse. This may be caused by the differences in the containing of biofilm-related genes, genetic make-up and physiological situation.