Detection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method

Abstract

A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.