Somatic Embryo from Basal Leaf Segments of Vanda tricolor Lindl. var. pallida

Abstract

Somatic embryogenesis is one of techniques in plant micropropagation. The induction of somatic embryogenesis through callus phase was done on Vanda tricolor Lindl. var. pallida. This study aimed to find out the effect of naphtalene acetic acid (NAA) and benzyl amino purine (BAP) in inducing somatic embryogenesis via callus on the basal leaf segments of Vanda tricolor Lindl. var. pallida. The half-strength of Murashige and Skoog (½ MS) medium with 1 % sucrose, incorporated with (0.02 mg · L –1 and 0.05 mg · L –1) NAA and also 0.01 mg · L –1 BAP were used in this experiment. The best medium for embryogenic callus formation and proliferation was 0.05 mg · L –1 NAA in combination with 0.01 mg · L –1 BAP. The formation of somatic embryos occurred 30 d after the calluses were cultured on to ½ MS without the addition of plant growth regulator and subsequently formed shoots.