Multiple shoot formation and plant regeneration of a commercially-useful tropical plant, Buchanania lanzan (Spreng)

Abstract

Buchanania lanzan (Spreng) is an endemic but vulnerable plant from the tropical region of India. A tissue culture technique for the rapid clonal multiplication of B. lanzan was developed. The decoated seeds were cultured on MS medium enriched with various concentrations of auxins and cytokinins alone or in combination. Combinations of Benzyl amino purine (BAP) and napthalene acetic acid (NAA) were found to be superior to BAP and Indole butyric acid (IBA). Murashige-Skoog (MS) medium supplemented with 22.2 μM of BAP and 5.37 μM of NAA promoted formation of the maximum number of shoots. Furthermore, MS medium containing 23.3 μM kinetin induced profuse rooting of the initiated shoots. We propose here that multiple shoot formation is one of the effective techniques for rapid clonal multiplication. Copyright © 2005 The Japanese Society for Plant Cell and Molecular Biology.