Antibacterial Effectiveness of Synthesized Copper Nanoparticles by Ultrasonication Assisted Method

Abstract

Antibiotic resistance among pathogenic bacteria has become a problem in the medical community. Copper nanoparticles (CuNPs) have become one method of combating antibiotic resistance in bacteria. The antibacterial activity of Synthesized Copper nanoparticles (CuNPs) from sigma 774103-5G against various species of harmful bacteria, including Staphylococcus aureus FNCC 0047, Escherichia coli FNCC 0091, and Salmonella typhimurium FNCC 0134, was determined in this research. CuNPs were optimized by ultrasonication in order to enhance the release of Cu ions into the environment. CuNPs ranging in size from 60 to 80 nm were employed. CuNPs' bactericidal impact can be assessed using a variety of methods, including the disk diffusion test, the Minimum Inhibitory Concentration (MIC), and the Minimum Bactericidal Concentration (MBC), and it can conducted with ultrasonification or non-ultrasonification respectively. The ultrasonication treatment was found to be efficient in boosting the antibacterial activity of all of the microorganisms examined. CuNPs demonstrated the best antibacterial activity against Staphylococcus aureus FNCC 0047, with a 23.7 mm inhibition zone diameter and a 37.5 g/mL MIC. The ultrasonication treatment was chosen for the preparation of CuNPs samples before further testing with the Alkaline Phosphatase Assay method, and the results showed that alkaline phosphatase enzyme as an indicator of cell damage was mostly detected in Staphylococcus aureus FNCC 0047 with a 4 hour exposure time. Membrane Integrity Staining with fluorescence microscopy viewing further revealed that cell damage increased as CuNPs exposure time increased.