2′, 3′-dideoxy-3′ amlnonudeo 5′ .triphosphates are the terminators of DNA synthesis catalyzed by DNA polymerases

Abstract

It is shown that 2′,3′-dideoxy-3′-aminonucleoside 5′-tri-phosphates with adenine, guanine, cytosine and thymine bases are effective inhibitors of DNA polymerase I, calf thymus DNA polymerase α and rat liver DNA polymerase β. The effect of the above-mentioned compounds is markedly higher than corresponding action of the well-known DNA synthesis inhibitors arabinonucleoside 5′-triphosphates and 2′,3′-dideoxynucleoside 5′-triphosphates. 2′, 3′ -dideoxy-3′ -aminonucleoside 5′-monophosphate residues incorporate into the 3′-terminus of the primer and terminate the DNA chain elongation. The possibility of using 2′,3′-dideoxy-3′- aminonucleoside 5′-triphosphates as terminators for DNA sequencing by the polymerization method is demonstrated. © 1984 IRL Press Ltd.