Green Assessment of Analytical Procedure for the Determination of Anti-Retro Viral Drugs by HPLC

Abstract

Aim: The proposed study was to establish a Greener method and validation, by green chromatography technique for combined anti-viral drugs, measuring greenness using various tools, which is one of the emerging developments in the analytical field. MATERIAL AND METHOD: The chromatographic separation is achieved by using C 18 (250x4.6, 5 μm) column by applying isocratic elution using the mobile phase containing Ethanol and Isopropyl acetate in the ratio of (55:45% v/v) with 1 mL/min flow rate. RESULT: The separation of drugs is achieved by using greener mobile phase.The Retention time of 1.854 min and 9.09 min for Ritonavir and Ombitasvir was found respectively. The regression coefficient (R 2) is 0.987 for the both drugs. Accuracy and precision is evaluated for the method and found be within the limit and the results were reproducible. Assessment of method was carried out using the three different tools. The developed method is anticipated to be eco-friendly, alternative to developed method UPLC method in regard to safe solvent, less toxic and less run time. The Proposed method was found suitable for the simultaneous estimation in their combined dosage form.