Monitoring the Utilization of Glyphosate Resistance Genes Based on Patent Analysis

Abstract

As a widely used herbicide in the world, glyphosate occupies an important position in the global agrochemical market. the first genetically modified glyphosate-resistant crop was successfully studied in the United States. Monsanto, as a giant in the agrochemical field, has extensive influence in the field of genetically modified glyphosate resistance. Phosphoenolpyruvate shikimate synthase ((EPSPS)) is a key enzyme that catalyzes the synthesis of EPSP from PEP and S3P in shikimic acid pathway. As an important prerequisite for branched acid synthesis in photosynthesis, shikimate synthase plays an important regulatory role. Glyphosate is the structural analogue of S3P, which competitively inhibits the enzyme activity of EPSPS, resulting in the interruption of shikimic acid pathway, which leads to the death of plants. According to their conserved domains, EPSPS can be divided into type I EPSPS and type II EPSPS, type I EPSPS sensitive to glyphosate, while type II EPSPS is inherently tolerant to glyphosate, and the two types of EPSPS have specific mutants, showing different degrees of sensitivity to glyphosate inhibition in the catalytic shikimic acid pathway. Based on China knowledge Network and other databases, mining the current types, sequence characteristics and transformation events of glyphosate-resistant genes will help our country to occupy a favorable position in the field of glyphosate-resistant transgenic and maintain the agricultural security of our country.