The curative effect of Bee Venom and Propolis on oxidative stress induced by γ-irradiation on blood and tissues of rats

Abstract

The biological effects of ionizing radiationresult in the formation of free radicals which are the main cause of cellulardamage. This study was aimed to detect the curative effect of some antioxidantsdrugs (Bee venom and Propolis) on the hematological, biochemical and histologicalparameters after exposed to γ-irradiation on the rats. Five groups of adultmale albino rats were established (n= 11). The first group (Gp. 1) was servedas normal group. Gp. 2 was exposed the whole body of rats to a single shot doseof gamma radiation (5Gy) and served as control group. Gp. 3 was injectedintraperitoneal (i.p.) daily with Bee venomat dose (5ml/kgbody weight). Gp. 4 was given orally Propolis daily at dose 300 mg/kg body weight (B.wt). Gp. 5 was administered of (5ml/kgbody weight) of Beevenom injected i.p. plus orally 300 mg/kg (B.wt) of Propolis, daily. All groups were treated after 8 hours exposure,for 40 day. Whole blood, serum and biopsy from liver and kidneys were collectedfor hematological, biochemical and histopathological examinations,respectivetly. In the irradiated group (gp. 2), hematotoxicity was determinedby a significant decrease in red blood cells (RBCs), white blood cells (WBCs),blood Platelets (PLTs), haemoglobin (Hb) and PCV value, as well as there are an elevation in serumALT, AST, ALP, LDH, creatinine and urea, associated with reduction in albuminand uric acid. Hepatotoxicity was recorded in a highly significant increasemalondialdehyde (MDA) with marked reduction in glutathione (GSH) levels,superoxide dismutase (SOD) and catalase (CAT) activities. In addition to,degenerative and necrotic changes showed in liver and kidneys. It could beconcluded that Bee venom and Propolis, clarified a modulatory role against thecellular damage produced by free radicals induced by ionizing radiation.