Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice

Abstract

This study evaluated the potential of bone marrow derived mesenchymal stem cells (MSCs) to regulate cytokines and remodel the lung induced by lipopolysaccharide (LPS; O-antigen). Materials & methods: A group of mice (n = 21) was inoculated intraperitoneally with one dose 0.1 ml containing 0.025 mg LPS/mouse, and another treated intravenously with one dose of labeling bone marrow derived MSCs at 7.5 × 105 cell/mouse 4 h after LPS injection. All animals were sacrificed on the 1st, 7th and 14th days post-injection . Results: MSCs increased the level of IL-10 with suppression of TNF-α, decrease of collagen fibers and renewal of alveolar type I cells, together with lung tissue remodeling. Conclusion: MSCs were shown to modulate inflammatory cytokines (TNF-α and IL-10) and to differentiate into alveolar type I cells, which prevented fibrosis in lung tissue from LPS-treated mice. Lay abstract: This study sought to confirm the remodeling effect of mesenchymal stem cells (MSCs) on lungs injury resulting from lipopolysaccharide infection, as lipopolysaccharide has an important role in acute lung injury pathogenesis. MSCs decreased the level of TNF-α and increased the level of antinflammatory cells (IL-10), leading to prevention of fibrosis with renewal of alveolar type I cells. This research aids our understanding of the utility of MSCs in chronic lung injury treatment.