Antimicrobial, Haemolytic and Antibiofilm Assay of Green Synthesized Silver Nanoparticles by Aqueous Extracts of Rubia cordifolia

Abstract

In the present investigation, a simple and effective green synthesis of silver nanoparticles uncoiled by using root and stem extracts from Rubia cordifolia (RC) followed by characterization and evaluation of their in-vitro antibacterial activity against human pathogenic bacterias. The biosynthesized silver nanoparticles had evaluated by using diverse physicochemical methods such as UV-Vis, XRD, DLS, FTIR, SEM, and EDS. These AgNPs showed an absorption peak in the range of 230-292 nm. AgNPs embraced a hexagonal or nearly spherical shape and the dimension of 105 and 64.6 nm under SEM analysis and additional substantiated by XRD results. A hexagonal wurtzite shape observed for the synthesized AgNPs, and the particle sizes were in the order of 32.16–32.17 nm as reckoned by DebyeScherer’s formula. The elemental analysis has performed using EDS authenticated the silver content of 69.06 and 54.48 for RCR-AgNPs and RCS-AgNPs, which testifies the explicit biosynthesis of nanoparticles. Further, the AgNps showed vital antibacterial potency against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aerogenes. The biosynthesized nanoparticles were report as a great antibiofilm agent against P. auriginosa and E. coli. Besides, the nanoparticle showed low to moderate cytotoxicity activity against human blood cells shows, the use of RC silver nanoparticles. Outcomes assert that biosynthetic AgNPs produce an exceptional potential alternative to present-day chemical compounds.