Long-term patterns of genomic P element content and P-M characteristics of Drosophila melanogaster in eastern Australia

Abstract

A latitudinal cline in characteristics associated with the P DNA transposable element is well known in eastern Australian populations of Drosophila melanogaster. In order to survey the long-term patterns of P-M system characteristics and genomic P element content, we established 292 isofemale lines from 54 localities in 1996-1997 and evaluated them for gonadal dysgenesis (GD) sterility and the ratio of KP to full-size P elements (KP/FP ratio). The results were compared to those from collections made in 1983-1986 and 1991-1994. Over 10-14 years, 1) the cross A GD scores of the northern-middle populations declined dramatically; 2) the clinal pattern of the cross A* GD scores did not change; 3) the latitudinal pattern of the KP/FP ratio did not change. The results suggest that only a few P elements determine P-M characteristics and that there has been selection for genomes with fewer active P elements, but not for a great change in proportions of size classes.