Induction of human fetal hemoglobin expression by adenosine-2',3'-dialdehyde

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Abstract

Background: Pharmacologic reactivation of fetal hemoglobin expression is a promising strategy for treatment of sickle cell disease and β-thalassemia. The objective of this study was to investigate the effect of the methyl transferase inhibitor adenosine-2',3'-dialdehyde (Adox) on induction of human fetal hemoglobin (HbF) in K562 cells and human hematopoietic progenitor cells.Methods: Expression levels of human fetal hemoglobin were assessed by northern blot analysis and Real-time PCR. HbF and adult hemoglobin (HbA) content were analyzed using high-performance liquid chromatography (HPLC). DNA methylation levels on human gamma-globin gene promoters were determined using Bisulfite sequence analysis. Enrichment of histone marks on genes was assessed by chromosome immunoprecipitation (ChIP).Results: Adox induced γ-globin gene expression in both K562 cells and in human bone marrow erythroid progenitor cells through a mechanism potentially involving inhibition of protein arginine methyltransferase 5 (PRMT5).Conclusions: The ability of methyl transferase inhibitors such as Adox to efficiently reactivate fetal hemoglobin expression suggests that these agents may provide a means of reactivating fetal globin expression as a therapeutic option for treating sickle cell disease and β-thalassemia. © 2013 He et al; licensee BioMed Central Ltd.

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He, Y., Rank, G., Zhang, M., Ju, J., Liu, R., Xu, Z., … Zhao, Q. (2013). Induction of human fetal hemoglobin expression by adenosine-2’,3’-dialdehyde. Journal of Translational Medicine, 11(1). https://doi.org/10.1186/1479-5876-11-14

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