Protective effects of compounds in bombax ceiba flower on benzo[a]pyrene-induced cytotoxicity

Abstract

The methanolic extract of the flower of Bombax ceiba was found to show protective effects on cytotoxicity induced by benzo[a]pyrene (BaP) in HT1080 cells. We therefore tried to examine and estimate the active constituents. We isolated 16 compounds from the extract, including four butyrolactones and two ascorbic acid derivatives, as well as mangiferin. Among the isolated compounds, a butyrolactone derivative, (-)-loliolide, and two flavonoids, kaempferol 3-O-β-Dglucopyranoside and quercetin 3-O-β-D-glucopyranoside, protected the cells against the BaP-induced cytotoxicity. Quercetin, the aglycone of one of the active constituents, showed a weaker effect than its glycoside. This is the first report of the protective effects of the methanolic extract of B. ceiba and its constituents on BaP-induced cytotoxicity.