Effect of water stress on antibacterial activity, Total Phenolic Content and Total Flavonoid Content of Clitoria ternatea

Abstract

The present investigation was aimed in determining the antibacterial activity of Clitoria ternatea that experiencing water stress treatment which comprises of positive control (watered normally for every 24 hours), negative control (no presence of water) and a treatment where C. ternatea receive water for every 48 hours. Agar disk diffusion assay had been utilized in order to investigate the measurement of zone of inhibition based on the methanolic leaf extract of C. ternatea for each treatment. Plus, calculation had been done for total phenolic content and total flavonoid content, to correlate between the length of inhibition zone. The results show that there is no presence of inhibition zone for all treatments which might be due to the low potency of the methanolic leaf extract, 0.02g/ml. C. ternatea that undergo every 48 hours of water presence hold the highest amount of total phenolic content whilst C. ternatea that encountered negative control of treatment possess the high amount of total flavonoid content. High quantity of phenolic and flavonoid content can be related with high amount of antioxidant capacity.