Determination of the rate of the glutamate/glutamine cycle in the human brain by in vivo 13C NMR

Abstract

Recent 13C NMR studies in rat models have shown that the glutamate/glutamine cycle is highly active in the cerebral cortex and is coupled to incremental glucose oxidation in an ≃1:1 stoichiometry. To determine whether a high level of glutamatergic activity is present in human cortex, the rates of the tricarboxylic acid cycle, glutamine synthesis, and the glutamate/glutamine cycle were determined in the human occipital/parietal lobe at rest. During an infusion of [I-13C]-glucose, in vivo 13C NMR spectra were obtained of the time courses of label incorporation into [4- 13C]-glutamate and [4-13C]-glutamine. Using a metabolic model we have validated in the rat, we calculated a total tricarboxylic acid cycle rate of 0.77 ± 0.07 μmol/min/g (mean ± SD, n = 6), a glucose oxidation rate of 0.39 ± 0.04 μmol/min/g, and a glutamate/glutamine cycle rate of 0.32 ± 0.05 μmol/min/g (mean ± SD, n = 6). In agreement with studies in rat cerebral cortex, the glutamate/glutamine cycle is a major metabolic flux in the resting human brain with a rate ≃80% of glucose oxidation.