HPLC analysis of glycosylated amino acids

Abstract

This work describes the standardization of HPLC analysis methodology for the identification and separation of the glycosylated amino acids (building blocks) 3, 4, 7 and 8 and their respective precursors. The glycosylated amino acids containing the sugar units β-D-GlcNAc (3 and 7) O-linked to threonine amino acids are involved in cellular signalling events, and the correspondent isomers 4 and 8, possessing α-D-GlcNAc units are related to O-glycans found in mucins of the parasite Trypanosoma cruzi. The developed methodology is advantageous and efficient as it permits the analysis of crude reaction mixtures, avoiding exhaustive purification processes. ©2006 Sociedade Brasileira de Química.