Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp

Abstract

Solid state fermentation was carried out using various agroindustrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50°C and 6 respectively. The enzyme was found to be thermostable at 70°C for about 2h without any salt. It showed stability at pH range 5-7. The metal ions as Na +, Ca ++, Mg ++ and Co ++ enhanced the enzyme activity.