Establishing an ex vivo porcine skin model to investigate the effects of broad-spectrum antiseptic on viable skin microbial communities

Abstract

Broad-spectrum antiseptics are widely used to prevent surgical site infection and as wound cleansing agents. The impacts of such agents on beneficial microbes in the skin microbiome are understudied. Here, we describe an ex vivo skin model to test the impacts of antiseptics or other topical agents on the healthy skin microbiome.