CHEMICAL CONSTITUENTS AND MOLLUSCICIDAL ACTIVITY OF IRIS PSEUDACORUS L. CULTIVATED IN EGYPT

Abstract

The molluscicidal activity of leaves and rhizomes extracts of Iris pseudacorus L. family Iridiaceae against Biomphalaria alexandrina snails were evaluated and proved activity. The successive extracts of leaves viz; n-hexane, chloroform, diethyl ether and ethyl acetate (LC 50 values ranged from 0.8~14.65 mg/L) were found to be more potent than those of rhizomes. Bioactivity guided fractionation of leaves extracts resulted in the isolation of (13) compounds. The structural determination of the isolated compounds was established upon the study of their physicochemical and spectral data including UV, IR, 1 H, 13 C-NMR and MS as well as comparison with the data reported in literature. These compounds were identified as: apigenin (1), luteolin (2), -sitosterol-3-O-glucoside (3), catechol (4), protocatechuic acid (5), gallic acid (6), (-)-epicatechin (7), iso-vitexin (8), vitexin (9), iso-orientin (10), orientin (11), and mangiferin (13). Reviewing the available literature, this is the first report regarding the determination of molluscicidal activity as well as isolation and identification of these compounds from Iris pseudacorus L.