Introduction and background: A tetravalent DNA vaccine for Dengue virus is under development but has not yet achieved optimal immunogenicity. Salivary glands vaccination has been reported efficacious in rodents and dogs. We report on a pilot study testing the salivary gland as a platform for a Dengue DNA vaccine in a non-human primate model. Materials and methods: Four cynomolgus macaques were used in this study. Each macaque was pre-medicated with atropine and sedated with ketamine. Stensen's duct papilla was cannulated with a P10 polyethylene tube, linked to a 500ul syringe. On the first two infusions, all macaques were infused with 300ul of TVDV mixed with 2 mg of zinc. For the 3rd infusion, to increase transfection into salivary tissue, two animals received 100uL TVDV mixed with 400uL polyethylenimine 1μg/ml (PEI) and the other two animals received 500uL TVDV with zinc. Antibody titers were assessed 4 weeks following the second and third infusion. Results and conclusions: SGRI through Stensen's duct is a well-tolerated, simple and easy to reproduce procedure. TVDV infused into macaques salivary glands elicited a significantly weaker antibody response than with different delivery methods.
CITATION STYLE
El Helou, G., Ponzio, T. A., Goodman, J. F., Blevins, M., Caudell, D. L., Raviprakash, K. S., … Sanders, J. W. (2020). Tetravalent dengue DNA vaccine is not immunogenic when delivered by retrograde infusion into salivary glands. Tropical Diseases, Travel Medicine and Vaccines, 6(1). https://doi.org/10.1186/s40794-020-00111-5
Mendeley helps you to discover research relevant for your work.