Synthesis and Preliminary Anti-Inflammatory and Anti-Microbial Evaluation of New 4,5-Dihydro-1H-Pyrazole Derivatives

Abstract

Pyrazolines are one of five nitrogen compound classes that have been linked to a variety of pharmacological activities. The current research involves the synthesis of new pyrazoline-aniline derivatives using chalcones as a key intermediate. Chalcones were synthesized by combining Furan-2-Carbaldehyde with various acetophenones via Claisen-Schimidt condensation. By refluxing substituted chalcones with hydrazine hydrate in ethanol and glacial acetic acid, 2-chloro-N-phenylacetamide and 2-chloro-N-(4-nitro-phenyl)acetamide were formed, which then react with the substituted aniline to yield 4,5-dihydro-1H-pyrazole derivatives as final products. The newly synthesized compounds were characterized using FT-IR and 1HNMR spectral data, and their anti-inflammatory, antibacterial, and antifungal activities were assessed. It was found that the incorporation of the pharmacophore pyrazoline into the substituted aniline improved the effectiveness of the compounds.