Análise seminal e padronização da coloração eosina-nigrosina em tamanduá-bandeira (Myrmecophaga tridactyla)

Abstract

Studies involving the reproductive aspects of the giant anteater (Myrmecophaga tridactyla) can be contributed to the preservation of this specie. The aim of these study was accessed the reproductive physiology and describe physical and morphological giant anteater’s sperm. We conducted three reproductive examination in two anteater maintained at the Federal University of Mato Grosso Zoo, Cuiaba/MT/Brazil. The method of semen collection used was electroejaculation. The volume average obtained was 2.62 ± 1.67 mL, the motility average was 50 ± 20% and the vigor was 2.17 ± 0.82. The pH mean was 7.29 ± 0.40. The sperm average concentration was 33.33 ± 12.52 x 106 sperm/mL. According sperm pathology we found 16.5% of larger defects, 20.5% of minor defects, amounting to 37 ± 4.2% of total defects. The preliminary tests of standardization of eosin-nigrosin staining proved to be effective to evaluate the integrity of plasma membrane of sperm in this species, in this research was 65 ± 7,77%.