The effect of different pre-treatments on carotenoid and phenol stability of Sargassum duplicatum methanol extract during storage

Abstract

Carotenoids and phenols, compounds contained in seaweed, have free radical scavenging activities. However, both compounds have low stability. Temperature, in particular, may affect the stability of both compounds. The aim of this study was to determine the effects of different pre-treatments, namely, blanching (high temperature) and freezing (low temperature), on maintaining the stability of methanol extracts of Sargassum duplicatum during storage at room temperature. The research method used was an experimental laboratory study using the basic design of a completely randomized design with a factorial pattern of 2×5, which was divided into the factors of processing temperature (high and low temperature) and storage time (0, 2, 4, 6, and 8 days). Blanching was carried out at a temperature of 95°C, whereas freezing was carried out at −27°C. The results showed that carotenoids, fucoxantin, and phenol compounds were more stable in the blanching treatment than in the freezing treatment. This study showed that blanching effectively delayed the degradation of carotenoids, fucoxanthin, and phenol content during storage at room temperature.