Comparative Evaluation on Antimicrobial Activity of Theobromine and Two Commercial Kids Fluoride Toothpaste – An Invitro Study

Abstract

Aim: Aim of this invitro study is to compare the antimicrobial activity of Theobromine containing kids tooth paste and two commercially available kids toothpastes. Materials and Methods: A chocolate based tooth paste, Theobromine (Group A) and two commercially available kids paste, Colgate kids (Group-B) and Kidodent (Group C) were used in this study. Freeze-dried stock culture of the strains of Streptococcus mutans, Lactobacillus acidophilus, Enterococcus faecalis were cultured in their respective selective media. Dentifrices were added to the wells puched out in the culture media and after incubation, the zones of inhibition were measured to find out the antimicrobial activity. Results: Theobromine showed a greater zone of inhibition which is statistically significant when compared to the other two pastes. Conclusion: Toothpaste containing theobromine containing toothpaste as shows higher antimicrobial effect against streptococcus mutans, lactobacillus acidophilus and enterococcus faecalis.