High-performance liquid chromatography-based characterization of fengycin produced by Bacillus amyloliquefaciens against Fusarium graminearum and Rhizoctonia solani

Abstract

A substantial loss of crop production worldwide is attributed to fungal phytopathogens. The most important economic pathogens among these fungal phytopathogens are Fusarium graminearum and Rhizoctonia solani, which cause a wide range of plant diseases. In the present study, Bacillus amyloliquefaciens secondary metabolite fengycin was identified by High-performance liquid chromatography (HPLC) and in vitro screened against the Fusarium graminearum and Rhizoctonia solani. Based on the HPLC result, fengycin was identified at 215nm wavelength, a retention time of 5-7 minutes, and a peak area of 3.914. The obtained results indicated that fengycin (1, 1/2, 1/4, and 1/8) concentrations have a significant effect (p<0.005) on the growth of F. graminearum and R. solani. The current study concluded that B. amyloliquefaciens secondary metabolites fengycin have a high potential to inhibit the growth of F. graminearum and R. solani.